Publications

Shyam lab publications

Ghag SA, de Campos VS, Murugan S, Herberg S, Shyam R. Decreased substrate stiffness leads to mitochondrial dysfunctions and Endothelial to Mesenchymal transition through Focal Adhesion Kinase activity in corneal endothelial cells. bioRxiv. 2025 Dec 2;. doi: 10.1101/2025.11.08.687366. PubMed PMID: 41279848; PubMed Central PMCID: PMC12637669.
Pattan HF, Murugan S, Shyam R, Tankam P. Enabling In Vivo Longitudinal Evaluation of Descemet's Membrane Thickness in Wild-type and FECD Mice Using Self-Referenced Optical Coherence Microscopy. Invest Ophthalmol Vis Sci. 2025 Sep 2;66(12):60. doi: 10.1167/iovs.66.12.60. PubMed PMID: 40996278; PubMed Central PMCID: PMC12476163.

Murugan S, de Campos VS, Ghag SA, Ng M, Shyam R. Characterization of a Novel Mouse Model for Fuchs Endothelial Corneal Dystrophy.Invest Ophthalmol Vis Sci. 2024 Apr 1;65(4):18. doi: 10.1167/iovs.65.4.18. PubMed PMID: 38587441; PubMed Central PMCID: PMC11005065.

Shyam R, Shu DY, Zuniga-Sanchez E, Vasudevan D. Editorial: Molecular mechanisms in ocular development and disease. Front Cell Dev Biol.2023;11:1244123. doi: 10.3389/fcell.2023.1244123. eCollection 2023. PubMed PMID: 37457294; PubMed Central PMCID: PMC10344605.

Liton PB, Boesze-Battaglia K, Boulton ME, Boya P, Ferguson TA, Ganley IG, Kauppinnen A, Laurie GW, Mizushima N, Morishita H, Russo R, Sadda J, Shyam R, Sinha D, Thompson DA, Zacks DN. AUTOPHAGY IN THE EYE: FROM PHYSIOLOGY TO PATHOPHYSOLOGY.Autophagy Rep. 2023;2(1). doi: 10.1080/27694127.2023.2178996. Epub 2023 Mar 1. PubMed PMID: 37034386; PubMed Central PMCID: PMC10078619.

Shyam R, Ogando DG, Bonanno JA. Mitochondrial ROS in Slc4a11 KO Corneal Endothelial Cells Lead to ER Stress. Front Cell Dev Biol.2022;10:878395. doi: 10.3389/fcell.2022.878395. eCollection 2022. PubMed PMID: 35557943; PubMed Central PMCID: PMC9086159.

Shyam R, Ogando DG, Kim ET, Murugan S, Choi M, Bonanno JA. Rescue of the Congenital Hereditary Endothelial Dystrophy Mouse Model by Adeno-Associated Viruse-Mediated Slc4a11 Replacement. Ophthalmol Sci. 2022 Mar;2(1). doi: 10.1016/j.xops.2021.100084. Epub 2021 Nov 23. PubMed PMID: 36051248; PubMed Central PMCID: PMC9432820.

Shyam R, Ogando DG, Choi M, Liton PB, Bonanno JA. Mitochondrial ROS Induced Lysosomal Dysfunction and Autophagy Impairment in an Animal Model of Congenital Hereditary Endothelial Dystrophy. Invest Ophthalmol Vis Sci. 2021 Sep 2;62(12):15. doi: 10.1167/iovs.62.12.15. PubMed PMID: 34533563; PubMed Central PMCID: PMC8458782.

Ogando DG, Shyam R, Kim ET, Wang YC, Liu CY, Bonanno JA. Inducible Slc4a11 Knockout Triggers Corneal Edema Through Perturbation of Corneal Endothelial Pump. Invest Ophthalmol Vis Sci. 2021 Jun 1;62(7):28. doi: 10.1167/iovs.62.7.28. PubMed PMID: 34190974; PubMed Central PMCID: PMC8826551.

Li S, Shyam R, Ogando DG, Bonanno JA. Bicarbonate activates glycolysis and lactate production in corneal endothelial cells by increased pH(i). Exp Eye Res. 2020 Oct;199:108193. doi: 10.1016/j.exer.2020.108193. Epub 2020 Aug 17. PubMed PMID: 32818510; PubMed Central PMCID: PMC7554184.

Ogando DG, Choi M, Shyam R, Li S, Bonanno JA. Ammonia sensitive SLC4A11 mitochondrial uncoupling reduces glutamine induced oxidative stress. Redox Biol. 2019 Sep;26:101260. doi: 10.1016/j.redox.2019.101260. Epub 2019 Jun 23. PubMed PMID: 31254733; PubMed Central PMCID: PMC6604051.

Shyam R, Vachali P, Gorusupudi A, Nelson K, Bernstein PS. All three human scavenger receptor class B proteins can bind and transport all three macular xanthophyll carotenoids. Arch Biochem Biophys. 2017 Nov 15;634:21-28. doi: 10.1016/j.abb.2017.09.013. Epub 2017 Sep 23. PubMed PMID: 28947101; PubMed Central PMCID: PMC5698089.

Gorusupudi, A., Shyam, R., Li, B., Vachali, P., Subhani, Y.K., Nelson, K., Bernstein, P.S.. Developmentally regulated production of meso-zeaxanthin in chicken retinal pigment epithelium/choroid and retina. Investigative Ophthalmology and Visual Science. 2016; 57(4):1853-1861. doi: 10.1167/iovs.16-19111.

Bernstein PS, Li B, Vachali PP, Gorusupudi A, Shyam R, Henriksen BS, Nolan JM. Lutein, zeaxanthin, and meso-zeaxanthin: The basic and clinical science underlying carotenoid-based nutritional interventions against ocular disease. Prog Retin Eye Res. 2016 Jan;50:34-66. doi: 10.1016/j.preteyeres.2015.10.003. Epub 2015 Nov 2. Review. PubMed PMID: 26541886; PubMed Central PMCID: PMC4698241.

Turturro, S., Shen, X., Shyam, R., Yue, B.Y.J.T., Ying, H.. Effects of mutations and deletions in the human optineurin gene. SpringerPlus. 2014; 3(1). doi: 10.1186/2193-1801-3-99.

Qiu Y, Shen X, Shyam R, Yue BY, Ying H. Cellular processing of myocilin. PLoS One. 2014;9(4):e92845. doi: 10.1371/journal.pone.0092845. eCollection 2014. PubMed PMID: 24732711; PubMed Central PMCID: PMC3986080.

Shen X, Ying H, Qiu Y, Park JS, Shyam R, Chi ZL, Iwata T, Yue BY. Processing of optineurin in neuronal cells. J Biol Chem. 2011 Feb 4;286(5):3618-29. doi: 10.1074/jbc.M110.175810. Epub 2010 Nov 8. PubMed PMID: 21059646; PubMed Central PMCID: PMC3030366.

Park B, Ying H, Shen X, Park JS, Qiu Y, Shyam R, Yue BY. Impairment of protein trafficking upon overexpression and mutation of optineurin.PLoS One. 2010 Jul 12;5(7):e11547. doi: 10.1371/journal.pone.0011547. PubMed PMID: 20634958; PubMed Central PMCID: PMC2902519.

Shyam R, Shen X, Yue BY, Wentz-Hunter KK. Wnt gene expression in human trabecular meshwork cells. Mol Vis. 2010 Jan 28;16:122-9. PubMed PMID: 20111673; PubMed Central PMCID: PMC2812481.

Koga T, Shen X, Park JS, Qiu Y, Park BC, Shyam R, Yue BY. Differential effects of myocilin and optineurin, two glaucoma genes, on neurite outgrowth. Am J Pathol. 2010 Jan;176(1):343-52. doi: 10.2353/ajpath.2010.090194. Epub 2009 Dec 3. PubMed PMID: 19959812; PubMed Central PMCID: PMC2797895.